We are seeking a motivated Ph D student with a background in biochemistry, cell biology or molecular biology. The nuclear pore complex (NPC) is a large cylindrical structure with multiple copies of over 30 different proteins named nucleoporins (NUPs). The NPC is embedded in the nuclear envelope (NE) at fusion sites of the inner and outer nuclear membrane where it facilitates nuclear-cytoplasmic transport of RNA and proteins. NPCs assemble in the intact nuclear envelope (interphase human cells or yeast cells with a closed mitosis) by an inside-out mechanism. NUPs become deposited from within the nucleus to the inner nuclear membrane, deform this membrane and eventually the inner and outer nuclear membranes fuse. We recently have identified with Brr6 and Brl1 two conserved integral membrane proteins that may play a role in nuclear membrane fusion during NPC biogenesis.

Recently, we identified two conserved integral membrane proteins, Brr6 and Brl1, which may play a critical role in nuclear membrane fusion during NPC biogenesis. This project will analyse the role of these proteins in the assembly of NPCs in yeast and human cells. The Ph D student will employ a variety of techniques, including biochemical approaches, lipid analysis, super-resolution microscopy (Min Flux), electron microscopy, CRISPR/Cas9 technology, and live-cell imaging to study NPC assembly.


Successful candidates will be part of an international team of Ph D students and postdocs that works at the forefront of scientific research. The Ph D student will be a member of the Heidelberg Biosciences International Graduate School (HBIGS) (www.hbigs.uni-heidelberg.de).


We offer:
The Ph D position is funded in the first instance for 3 years with starting date based on mutual agreement (possible from Nov./Dec. 2024). The remuneration is based on TV-L E13.


Application deadline: October 15, 2024


Interested?
Please send applications (CV, motivation letter, 2-3 referees and a copy of Bachelor and Master transcripts) to Elmar Schiebel (schiebel.elmar@zmbh.uni-heidelberg.de).


Mailing address: Prof. Elmar Schiebel, ZMBH, Heidelberg University, Im Neuenheimer Feld 345, D-69120 Heidelberg, Germany.


For literature on this topic, please follow this link to our website:
www.zmbh.uni-heidelberg.de/schiebel/open.html.


Heidelberg University stands for equal opportunities and diversity. Qualified female candidates are especially invited to apply. Persons with severe disabilities will be given preference if they are equally qualified. Information on job advertisements and the collection of personal data is available at www.uni-heidelberg.de/en/job-market.